Pedroia sustained what the team announced was a mild sprain of the left wrist and was replaced in the bottom of the second by Angel Sanchez.
The injury occurred when Pedroia made a diving stop of a smash hit by Denard Span, the Twins' first hitter of the night. Pedroia scrambled to his feet but threw wildly to first and was charged with an error. He finished the inning, but when the Red Sox took the field in the second, Sanchez was in the game.
Boston manager Terry Francona said that Pedroia had "limited" swelling on the top of his wrist and that he will have X-rays Wednesday as a precaution.
The Twins' doctor took a look at Pedroia's wrist, but no X-rays were taken at Hammond Stadium Tuesday night.
"I don't think there's a problem. He might be a little sore tomorrow," Francona said. "Hopefully that's all it is."
The Red Sox said Pedroia's status was day to day, and Francona said he was not scheduled to make the trip to Bradenton to play the Pittsburgh Pirates on Wednesday afternoon.
Red Sox starting pitcher Clay Buchholz said he noticed Pedroia "wincing a little bit" in the dugout after the inning. "Asked him if he was all right,'' Buchholz said, "and he said he would be OK.''
Gordon Edes covers the Red Sox for ESPNBoston.com. Information from The Associated Press contributed to this report.