The Tigers also announced Friday outfielder Clete Thomas was going to the DL, retroactive to June 4, and right-hander Aquilino Lopez was on the bereavement list. Detroit also added outfielder Brent Clevlen.
Santiago is sidelined with a separated left shoulder and Thomas is out with a sprained right ankle.
Bautista was 0-1 in 6 1/3 innings and struck out seven. He was put on the disabled list May 3 with right shoulder tendinitis.
Hollimon, whose contract was purchased from Triple-A Toledo, hit .250 with 12 homers in 44 games for the Mud Hens. Clevlen, whose contract was recalled from Toledo, had a .324 batting average with 13 homers and an International League-leading 55 RBIs.
The Tigers opened a homestand against the Cleveland Indians on Friday night with the third-fewest victories in the AL.