His lawyer requested the postponement. The Nebraska hearing had been scheduled for Tuesday and was reset for Wednesday.
"I'm going to show up and we're going to take care of business," Chamberlain said Tuesday after pitching 5 1/3 innings in New York's 6-3 win over the Reds.
Chamberlain had been granted delays earlier this month and in December and January.
Chamberlain, who played for Nebraska in college, is charged with first-offense drunken driving and having an open alcohol container. He was pulled over Oct. 18 by police on the outskirts of Lincoln.