Jackson, who leads the Eagles with eight touchdowns this season, suffered a concussion in last week's win over the Washington Redskins.
"He felt pretty good today, but he's not all the way back yet," Eagles coach Andy Reid said. "He still has some normal headaches. He's just not ready yet."
Jackson, wide receiver Kevin Curtis (knee), running back Brian Westbrook (concussion) and linebacker Akeem Jordan (knee) were ruled out by Reid after Friday's practice. Curtis has been out since the second week of the season.
Tight end Brent Celek, who suffered a sprained thumb in last week's win, practiced this week and Reid said he should play.
Westbrook will miss his third straight game since suffering his second concussion of the season in San Diego. The running back has missed six games.
Westbrook visited doctors in Pittsburgh this week and the news was somewhat encouraging. Still, he remains out indefinitely.
"[Trainer] Rick [Burkholder] has been in contact with the doctors in Pittsburgh," Reid said. "We'll see what happens next week. He's been feeling better and that's a plus."
Jordan is out for the fourth straight week. The starting weakside linebacker returned to practice on a limited basis Wednesday and Thursday, but was not on the field Friday.
"He's just not quite ready," Reid said. "He had a little bit of swelling there, so we backed off a bit."
Jackson's loss also will be felt in the return game.
Jason Avant, who made two huge catches against the Redskins, including a 46-yarder during a game-tying drive in the fourth quarter, is expected to start opposite rookie Jeremy Maclin at wide receiver.
Seldom-used Reggie Brown, who has just three receptions this year, will be the third receiver.
Maclin also will replace Jackson as the team's primary punt returner. Jackson has averaged 15.5 yards on 21 returns this year, while Maclin has lost four yards on three returns.
"It's important when someone goes down for the next person to step up and play well," Reid said. "That's always been the case."
After Jackson went down last week, Maclin came up big on the Eagles' game-winning drive against Washington, making two catches that led to David Akers' field goal to win, 27-24. He's ready to do so again.
"Chances are the ball will come my way more, obviously. When the opportunity arises you have to seize the moment," the rookie first-round pick said. "In the NFL, careers are short. You have to go for it while you can."
Philadelphia (7-4) is one game behind the Dallas Cowboys in the NFC East. Atlanta (6-5) is five games behind the 11-0 New Orleans Saints -- who can clinch the NFC South this week -- but the Falcons are in the thick of things for a wild-card berth.