Coach Chan Gailey said Lynch looked good in returning to practice Tuesday for the first time since hurting his ankle in a loss to Washington.
"It didn't look like there was any residual effect, so we're looking forward to watching him Thursday night," Gailey said.
Lynch's return means the Bills will have two-thirds of their running back rotation healthy. First-round draft pick C.J. Spiller has carried most of the running load the past two preseason games, and Fred Jackson has been out since breaking a bone in his left hand against the Redskins.
Wearing a brace around his hand, Jackson practiced Tuesday on a limited basis. Jackson took part in individual drills and also tested his injured hand by catching a few soft passes.
"I felt like I was able to go full-go, but I still can't hold the ball in my left hand like I wanted to with this brace that I have on," Jackson said. "Hopefully, next week, I could come out and do some more stuff."
Jackson is a long shot to be ready for Buffalo's season opener against Miami on Sept. 12.
Coming off his first career 1,000-yard season, Jackson opened training camp as the Bills starter and was expected to get a fair share of carries despite the addition of Spiller.
Spiller has proven to be the Bills' offensive star this preseason. In the past two games, he has 22 carries for 106 yards and three touchdowns, and three catches for 35 yards.
Gailey said veteran linebacker Kawika Mitchell was absent from practice due to an injury. Gailey would only describe the injury as "a little issue" which the player was having checked.
Cornell Green, who's projected to start at right tackle, did not practice after hurting his knee in a win over Cincinnati on Saturday. Gailey said Green would be game-time decision.
Rookie offensive tackle Ed Wang practiced on a limited basis for the first time since hurting his left hand Aug. 2. Wang had his hand extensively wrapped and there's still no timetable on when the fifth-round pick out of Virginia Tech could play.