Broncos coach Josh McDaniels said Friday that Moreno will be out after sustaining "a little something" in practice that "he won't be able to play through." McDaniels didn't elaborate but the official injury report said Moreno had injured a hamstring.
Moreno has scored a touchdown in each of the 1-1 Broncos' games this season, totaling 111 yards rushing on 39 carries.
Maroney, who has been nursing a thigh injury, participated in a full practice Thursday.
"You've just got to keep on moving on," quarterback Kyle Orton said about the latest injury news. "There's nothing you can do or say about it. We've been dealing with this stuff all year. And whoever's in the huddle, we expect them to perform."
This is just the most recent injury for Moreno. The starting tailback pulled his right hamstring the opening day of training camp and didn't return until Aug. 31, saving his on-field debut until the regular season. He has gained just 111 yards on 39 carries for a 2.8-yard average this season.
Buckhalter hurt his neck and back in the first team drill of training camp and was sidelined for three weeks. Buckhalter played in the preseason finale and has 17 carries for 34 yards with a TD during the regular season.
"Without going through a preseason and getting banged around and stuff for that month, my preseason's kind of been the last two games we played," Buckhalter said. "So just getting the chance to get the rust off, I'm kind of feeling like myself right now, especially late in the week."
Champ Bailey, who has a foot injury, is listed as a game-time decision for the Broncos.
Information from ESPN.com senior NFL writer John Clayton and The Associated Press was used in this report.