Pavel Kubina, Simon Gagne ruled out
Both players suffered head injuries in Game 1, a 4-2 win for the Lightning.
Jones has been physically able to play for a few days, but Tampa Bay coach Guy Boucher said he was reluctant to tinker with his lineup during the latter stages of the Pittsburgh series because the team was doing well.
Gagne and Kubina are considered day to day.
"There's a possibility for maybe one of them Tuesday, maybe both. But it's day to day," Boucher said.
Carlson missed the third period of Game 1 with what appeared to be a back issue, but skated Sunday morning.
Knuble, who has missed the past three games with an apparent hand injury, returned after Capitals coach Bruce Boudreau said earlier in the day that he doubted the forward would be available for Game 2.
Scott Burnside covers the NHL for ESPN.com.